S80550-5mg Zotarolimus 221877-54-9 98% 5mg 1040.00 现货

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商品详情

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产品描述： Zotarolimus, an analogue of rapamycin, inhibits FKBP-12 (IC50= 2.8 nM).
靶点： mTOR
体内研究： Zotarolimus potently inhibits adjuvant DTH(ED50=1.72 mg/kg/day), EAE(ED50=1.17 mg/kg/day), and cardiac allograft rejection(ED50=3.71 mg/kg/day)
细胞实验： Cell proliferation is assayed by measuring tritiated thymidine incorporation in vitro. Human coronary artery cells (hCa) are seeded into tissue culture flasks for expansion and applied to 96-well plates at desired density in complete media (5000 hCaSMC; 10 000 hCaEC). After 2 days, complete media is replaced with incomplete media to synchronize cells and induce G0 state. Two days later, incomplete media are removed and replaced with complete media (serum/growth factors) to induce G0 to G1 transition. Complete media also contain drug at desired concentrations to determine its effects on cell proliferation. On day 7, 3H-thymidine is added to cells to monitor DNA synthesis, and cells are harvested after overnight incorporation of radioactivity. After an incubation period of 72 h, 25 μL (1 μCi/well) of 3H-thymidine are added to each well. The cells are incubated at 37°C for 16-18 h to allow for incorporation of 3H-thymidine into newly synthesized DNA and the cells harvested onto 96-well plates containing bonded g
动物实验： Male Sprague-Dawley rats was administrate by intravenous or oral Zotarolimus（2.5 mg/kg）dissolved inethanol: propylene glycol: cremophor EL: D5W vehicle (20: 30: 2: 48, by volume).
参考文献：
1.Chen YW, et al. J Cardiovasc Pharmacol, 2007, 49(4), 228-235. 2.Garcia-Touchard A, et al. Eur Heart J, 2006, 27(8), 988-1993.
溶解性： Ethanol:93 mg/mL (96.3 mM) H2O:<1 mg/mL DMSO:93 mg/mL (96.3 mM)
保存条件： 2-8℃
配置溶液浓度参考：

	1mg	5mg	10mg
1 mM	1.035 ml	5.175 ml	10.35 ml
5 mM	0.207 ml	1.035 ml	2.07 ml
10 mM	0.103 ml	0.517 ml	1.035 ml
50 mM	0.021 ml	0.103 ml	0.207 ml

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